Morning Tea

Afternoon Tea

Correlative microscopy is a powerful and sophisticated microscopy approach that enables detailed subsurface sample analysis. As material science research advances, there is a need to understand the effect of microstructure and properties of a sample at different location and length scale. In view of this, a multi-scale imaging and analysis workflow that ranges from the macro- to sub-nanometer length scale has been developed. The workflow presented here aims to provide navigational guidance to connect modalities necessary for multi-scale imaging and analysis. Application examples in battery research, additive manufacturing and life sciences will be discussed to demonstrate how this workflow is enabling scientific advances in various research fields. 

Are you new to the world of AI for image analysis and you don’t know how you can best leverage these tools? Join this techtalk to learn about the different AI tools for image analysis, what are the advantages and disadvantages of conventional machine learning versus deep learning and how to get started training your own AI models without any programming knowledge.

Morning Tea

Afternoon Tea

The implementation of more biologically relevant in vitro models requires an evolution of both 3D culture methods and imaging techniques. This techtalk will explore cutting-edge bioprinted 3D cultures and tailored imaging workflows that address these challenges, enabling researchers to harness the full potential of complex in vitro models for biomedical research.

Electron channelling contrast imaging (ECCI) is an SEM based technique for observation of extended crystal lattice defects like dislocations and stacking faults. It exploits the dependence of the backscatter electron intensity on crystal orientation and atomic order. For ECCI a crystalline sample is observed with the backscattered electron signal. The basic principle of contrast formation is that electrons channel into a crystal lattice when the incident beam hits the lattice along the Bragg angle of a set of crystal planes. In this case very few electrons are backscattered, and the observed crystal appears dark. Under this condition, every defect that disturbs the order of the lattice planes leads to backscattering and are visible in the ECC image as bright features in a dark grain. In this presentation, we will present the working principles of the technique and demonstrate its capability and advantages through several examples of observations on a variety of materials ranging from superalloys, high strength steels and ceramics. 

Morning Tea

Afternoon Tea

Structured Illumination Microscopy (SIM) has become the most popular super-resolution method for live cell imaging. In classical SIM using one-dimensional line gratings resolution enhancement is up to 120 nm. The necessity to take around 15 images to reconstruct the super-resolved image, however, limits the dynamics.  Over the last years two-dimensional lattice patterns have been developed leading among others to an increase in signal-to-noise levels and improved penetration depths. We will present how the photon budget can be used either to reduce the number of required raw images or to enhance lateral resolution well below 100 nm. Thus, structuring with a two-dimensional lattice allows to study the interplay of organelles and other macromolecular structures in real time with unprecedented resolution. 

Modern materials research addresses a broad range of questions in diverse material systems ranging from polymers, metals, and ceramics to functional and composite materials with specific applications in areas like energy storage and conversion. Recent advancements in laboratory scale 3D X-ray microscopy have opened the door for new insights and experimental possibilities across all these areas. This talk will discuss these advancements within the context of the modern analysis laboratory using relevant examples to illustrate the concepts.